Dengue fever is one of the clinically important diseases in the world in terms of morbidity and mortality. Little is known about dengue infection in the Yemen. This work aimed at studying the presence/absence of dengue fever in the Yemen. The aim was performed through the determination of the frequency of dengue fever infection among patients presenting to Sana’a hospitals with pyrexial illnesses and the determination of any dengue-sero-positivity among apparently healthy individuals in six selected geographical locations in the Yemen. The study also aimed at determining the predisposing factors for dengue infection among dengue patients or apparently healthy individuals with asymptomatic infection with dengue.
A total of 158 blood specimens from 158 pyrexial patients and 716 blood specimens from 716 apparently healthy individuals were collected for a case finding study and epidemiological study, respectively. The specimens were drawn from individuals at all ages, with different economical and educational levels and representing different environments and geographical areas. Serological examination was performed to detect IgM and IgG antibodies against the four dengue virus serotypes (DEN 1-4).
All specimens in both parts of this study, the case finding study and the epidemiological study, were negative for both anti-dengue IgM and IgG antibodies. The pattern of infectious diseases in the case finding study showed that infections were responsible for 43.6% of the total cases. 56.4% of the total cases were undiagnosed. Among the infectious diseases the percentage of patients with malaria was 13.9%; typhoid, 12.0%; brucellosis, 8.2%; suspected viral infections other than dengue, 7.0% and tuberculosis, 2.5%.
This study concludes that the Yemen is free from dengue viruses up to the time of this study.
First of all I would like to express my gratitude to ALMIGHTY ALLAH for His generous help in accomplishing this work.
I am indebted to my supervisor Dr. HASSAN ABDUL-WAHAB AL-SHAMAHY Head, Department of Medical Microbiology, Faculty of Medicine and Health sciences, Sana’a University, for his supervision, valuable advice and suggestions, encouragement and continuous interest from the start to the end of this work.
I am gratefully indebted to my supervisor Dr. MOHAMMED ABDO M. NASHER, Associate Professor of Medical Microbiology, Faculty of Medicine and Health Sciences, Sana’a University, for his valuable advice and suggestions, useful constructive criticism and extremely unlimited efforts and intensive correction of the manuscript.
My thanks are due to Dr. SALEH F. AL-SALAMI for his valuable advice and suggestions.
I would like also to express my deepest thanks to my brother MOHAMMED YOUSIF MAI for his great help and cooperation throughout this work.
Many thanks to all those who helped me in collecting blood specimens in this study.
I would like to thank Dr. WILLIAM SCOTT McCULLOUGH for his help in communication with the company which bought the test kits for me.
I will remain much obliged and grateful to all my lecturers, who did not save any effort to advise and guide me through the different stages of my tuition.